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Free Download Full Torrent Games for PC,PS3,PS4,XBOX,XBOX ONE,PS Vita,Nintendo Switch,.. Star Wars. Jedi. Fallen. Order. PC Full Torrent. Star Wars Battlefront 2.-terminal portion of MAp44. Data shown are the means and SEMs of fluorescence intensity of at least three different experiments. ns, *P* \> 0.05; \**P* ≤ 0.05; \*\**P* ≤ 0.01.](nihms622811f2)#F2


![Generation of chimeric proteins containing the C-terminal regions of two divergent mammalian ERAD components. (A) Schematic diagram of chimeric proteins. (B--D) HeLa cells were transfected with plasmids expressing chimeric proteins, together with plasmids expressing Ub^G76V^-GFP (B, C) or HA-Ub (D). All constructs contained a C-terminal HA tag and were cloned into the pCMV-Tag2 vector. (E) HeLa cells were transfected with plasmids expressing HA-ubiquitin, which co-expressed a control, myc-tagged mutant ERAD E3 ligase, or three genes encoding chimeric proteins. Myc-ERAD E3 ligase was cloned into the pCMV-Tag2 vector. Immunoprecipitated proteins were separated by SDS-PAGE and the membrane was blotted with anti-ubiquitin. In the adjacent panel, the membrane was stained with 0.1% Ponceau-S. (F) Quantification of ubiquitinated HA-ubiquitin immunoreactive band intensity and myc-tagged mutant ERAD E3 ligase-immunoreactive band intensity was determined as in (E). Data shown are the means and SEMs of fluorescence intensity of at least three different experiments. ns, *P* \> 0.05; \**P* ≤ 0.05; \*\**P* ≤ 0.01; \*\*\**P* ≤ 0.001.](nihms622811f3)#F3


![Generation of chimeric proteins containing the C-terminal regions of mammalian and yeast ERAD components. Schematic diagram of chimeric proteins. The sizes of DNA fragments